Coding

Part:BBa_K3036006:Design

Designed by: Di Huang   Group: iGEM19_BNU-China   (2019-10-08)


GFP with degradation conduction tag RepA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 692


Design Notes

None.


Source

GFP is derived from jellyfish Aequeora Victoria and we extracted its certain sequence from 2019 Distribution kit. RepA tag is derived from proteins which could be recognized by an enzyme relevant to degradation. We add this tag to GFP by elongating the primers from N-terminal.

References